Exploring urine sediments as a non-invasive method for DNA methylation detection in bladder cancer

Abstract Background The main epigenetic event occurring during the bladder carcinogenesis process is DNA methylation, affecting genes involved in various metabolic pathways and cell regulation. use of biological fluids such as urine sediments could be used a non-invasive approach to enhance cancer management. In this study, we aim determine promoter methylation status panel on tumor biopsies evaluate usefulness samples for assessment. Methods Using methylation-specific PCR technique, explored hTERT , TWIST1 VIM NID2 40 their paired from Moroccan patients. Results tumors showed hypermethylation frequency individual 90%, 85%, 62.5% 72.5% TWIST1, hTERT, genes, respectively. Interestingly, specificity detection was 100% sensitivity detect reached 91.7%; 97.1%; 84% 82.8%, Conclusions Our results clearly show that assessment highly specific has high sensitivity. Furthermore, would useful its potential association with development.